Histone H2A.X Antibodies Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX.

Histone H2A.X is a variant histone that represents approximately 10% of the total H2A histone proteins in normal human fibroblasts (1). H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1).

Histone H2A.X Antibodies Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. Phosphorylated H2AX (gamma-H2AX) is essential to the efficient recognition and (or) repair of DNA double strand breaks (DSBs), and many molecules, often thousands, of H2AX become rapidly phosphorylated at the site of each nascent DSB. An antibody to gamma-H2AX reveals that this highly amplified process generates nuclear foci. 2010-02-04 Histone H2A.X is a variant histone that represents approximately 10% of the total H2A histone proteins in normal human fibroblasts (1). H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1). Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation.

Balajee, A. S. & Geard, C. R. Replication protein A and gamma-H2AX foci assembly is triggered by cellular response to DNA double-strand breaks. Exp Cell Res 300, 320–334 (2004). CAS Article H2AX is a reported alias name for the human gene H2AFX, or 'H2A histone family member X'. The 143-amino acid protein is a member of the Histone H2A family. The cellular localization is predicted to be … 2017-02-03 A gamma-H2AX kit and antibody allows the assessment of DNA damage and DNA repair for ELISA based assays, immunohistochemistry or flow cytometry. In the detection of genomic damage, gamma-H2AX is especially important in research involving cancer treatment and … induce its function.

A700-053. 100 µl (50+ tests) $389.

Anti-gamma H2A.X (phospho S139) antibody [EP854 (2)Y] (ab81299) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – confirmed specificity through extensive validation.

H2AX also plays an active role in supplying the active repair proteins. γH2AX participates in DSB repair. This protein serves as a signal and target of phosphorylation in the spreading phase. Our preliminary tissue microarray study demonstrated that gamma-H2AX, an antibody that specifically reacts with phosphorylated histone H2AX, stained many primary RCC strongly and did not stain HCC or ACC, prompting us to evaluate its utility in these tumors and to compare it with RCC-Ma.

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4).

Cat.No. 10856-1-AP. Invitrogen Anti-Phospho-gamma-H2AX (Ser139) Recombinant Monoclonal (RM224), Catalog # MA5-33062.

An antibody can be raised against gamma-H2AX which can therefore be visualized by immunofluorescence through secondary antibodies. The detection and 世界最大級の抗体製品数を取り扱うNovus Biologicals社のgamma H2AX [p Ser139]に対する抗体(anti-gamma H2AX [p Ser139] | antibody gamma H2AX [p gamma H2AX [p Ser139] Antibody Novus Biologicals catalog: NB100-384 total number of products: 7. domestic rabbit polyclonal (5B12) reactivity: human X Antibody (Ser 139) is a monoclonal phospho-specific anti-Histone H2A antibody recommended for WB, IP and IF. Cited in 38 The phosphorylated γ- H2A. Reactivity, Human, Mouse.
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Visit Bethyl.com for all your antibody needs.

The epitope maps to a region surrounding phosphorylated serine 139 of human histone H2AX. Reactivity Human, Mouse, Rat, Canine Host Rabbit Isotype IgG Vial size 0.1 ml Concentration 1.0 mg Histone H2AX: Products. Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX).
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Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139)

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX).

Phosphorylated H2AX (gamma-H2AX) is essential to the efficient recognition and (or) repair of DNA double strand breaks (DSBs), and many molecules, often thousands, of H2AX become rapidly phosphorylated at the site of each nascent DSB. An antibody to gamma-H2AX reveals that this highly amplified process generates nuclear foci.

Polyclonal Antibody for studying H2AX (Ser139) phosphate in the Chromatin Regulation / Epigenetics research area. p-H2AX antibody has to be used in Western blotting. Note that Western procedure for histons is a bit different from that for other proteins. First, p-H2AX indicates that there is DNA damage H2AX belongs to the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX is phosphorylated on Ser-140 to form gamma-H2AX in response to DNA double-strand breaks. Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit has been used in immunofluorescence and western blotting.

H2AX, H2a.x, H2afx, H2A/X.